Questions about hydroponic growth

mmelan at pattie.wellesley.edu mmelan at pattie.wellesley.edu
Wed Apr 28 15:49:08 EST 1993


Dear Hon-Ming,

        We have consistently gotten large quantities of clean, sterile root
tissue by growing plants in "liquid culture".  All you need to do is sow
approximately 10-20 Chlorox sterilized seed in 50 ml Murashige and Skoog
media (make your own or use GIBCO's) + vitamins + 3% sucrose in a 250 ml flask.
(The media is sterilized of course)  The flasks are placed on a rotary shaker
and constantly shaken at 150 rpm under low light (room lights are fine).  The
MS media should be changed weekly and in 2-3 weeks you should have a LOT of
root tissue in the flasks.  The shoot tissue will look strange and you can
decide whether or not to use it.  Hope that this is helpful.

===============================================================================
Melissa Melan                           mmelan at lucy.wellesley.edu
Department of Biological Sciences
Wellesley College
Wellesley, MA  02181
================================================================================



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