Genome workshop

[Schaeffner at de.d400.uni-muenchen.CHEMIE.BIOCHEMIE]

Arabiflora population!
Sorry, I just forgot a comment to the mailed program of the Arabidopsis genome
workshop at the Columbus meeting:
I am looking to interesting discussions at the workshop. There will be short
contributions as listed to leave enough time for your ideas and questions!

If you have further questions regarding the workshop ,
please contact Pablo Scolnik, DuPonzt, Wilmington, De E, USA
phone 302-695-7035
email scolnipa at esvax.dnet.dupont.com

Tony Schaeffner
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WORKSHOP ON GENOME RESEARCH
_________________________________________________________________

at Arabiflora, Columbus, OH
Aug. 22, 1.00 p.m. - 4.30 p.m.
_________________________________________________________________


-   comprehensive overview over state of the art
-   short statements (5-10 min) to various problems, techniques,
    advances
-   considerable part of time for open discussion at each topic



Physical map, contigs
_____________________

P.Dunn                  Ordering of YACs by anchoring
H.Goodman (tentatively) Progress on physical map
R.Schmidt               Contigs on chromosome 4: Conclusions
                        and consequences
M. Zabeau               Physical mapping using AFLP

Discussion


ESTs, sequenced genes
_____________________

Sequencing:
H.Hoefte                Current status of French EST effort
T.Newman                talks already at main meeting
                        (will be present for discussion)

Functions:
K.Feldmann              Sequenced genes vs.T-DNA lines
B.Reiss                 New approaches towards gene targeting?

Discussion


Mutation-based genome research
______________________________
J.Vizir                Saturating the top arm of chromosome 5
                       with mutations
B.Osborne              Cre-lox induced deletions, inversions,..
D.Meinke               Saturating the map with embryonic
                       mutations

Discussion


Molecular markers
_________________
T.Schaeffner            Arabidopsis RFLP mapping set (ARMS)
J.Glazebrook            Co-dominant amplified polymorphic
                        sequences (CAPS)
D.Preuss                PCR based markers
P.Scolnik               New approaches to RAPDs

Discussion


The endgame, the last steps
___________________________

Fine-mapping strategies:
Jo Putterill            Flanking emb for selection of
                        recombinants
B.Whittier              Thermal assymetric interlaced (TAIL) PCR
                        for walking
J.Leung                 Lessons from position cloning of abi3

Complementation:
R.Kuhn/ R.Ludwig        Transformation competent cosmid library
Transformation:
Annette Vergunst        Factors influencing transformation
                        efficiency

Discussion