Postdoctoral position in plant molecular genetics

Ljerka Kunst kunst at UNIXG.UBC.CA
Tue Aug 10 13:25:41 EST 1993


     A postdoctoral postition is available immediately in the laboratory
of Ljerka Kunst, Department of Botany, University of British Columbia to
work on the molecular genetics of seed lipid metabolism in Arabidopsis.

     The major objective of my research program is to elucidate molecular
mechanisms involved in the formation of very long chain fatty acids
(VLCFAs), such as eicosenoic (20:1) and erucic (22:1). These fatty acids
are formed in developing oilseeds by sequential addition of acetate units
to the carboxyl end of the preformed C18 fatty acids. Virtually nothing is
known about the enzymes involved in the elongation reactions, or the
functional significance of VLCFAs in seed storage lipids. We are using the
genetic approach in Arabidopsis thaliana to determine the number of
enzymes involved in the elongation process, obtain information about their
specific roles and compartmentation, identify important regulatory steps
in the VLCFA biosynthetic pathway and clone the genes of interest.

     The successful candidate will be involved in cloning and molecular
characterization of the FAE1 gene identified by a mutation in Arabidopsis
(Kunst et al. Plant Physiol. Biochem. 1992, 30, 425-434) using two
different approaches: map-based cloning and heterologous probing. In
addition, the candidate will have an opportunity to participate in genetic
and biochemical characterization of several new mutants with altered VLCFA
composition. Applicants should have extensive experience in molecular
biology and a good understanding of genetics. Funding is available for a
year at $24,000/yr, with a possibility of extension of up to three years.
If interested, please send a cover letter oulining research experience, a
curriculum vitae and names and addresses of 3 referees as soon as possible
to Ljerka Kunst, Department of Botany, University of British Columbia,
6270 University Blvd., Vancouver, B.C. V6T 1Z4, Canada; FAX
(306)822-6089;e-mail kunst at unixg.ubc.ca.

     For additional information I can be reached at (306)822-2351, or
contacted by leaving a message on Bulletin Boards during Canadian Society
of Plant Molecular Biology meeting (Toronto August 14-17), or the
Arabidopsis meeting (Columbus, Ohio August 19-22).





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