cellular fractionation

Barb Moffatt moffatt at SCIBORG.UWATERLOO.CA
Thu Aug 12 17:51:50 EST 1993


Dear Arabidopsis network readers:
        I would like to perform cell fractionation experiments to determine
the subcellular localization of a soluble enzyme, using Arabidopsis leaf
tissue. I was going to start from protoplasts. Does anyone out there have a
good protocol for obtaining organelle fractions, perhaps by differential
centrifugation or a gradient centrifugation. Any suggestions would be much
appreciated.
Thanks.
Alicia Smith
henderso at sciborg.uwaterloo.ca




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