Searching DNA isolation methods from plant tissue culture

Maren Jacob [Biologie] maren at rhrk.uni-kl.de
Tue Dec 21 05:30:58 EST 1993


I'm working with transformed Phaseolus vulgaris callus cultures
and I want to start with molecular DNA analysis (southern/dot
blot).I can't obtain enough DNA (not more than 1.5 ug/5 g fresh
weight) from a limited amount of callus material with conventional
DNA isolation methods (Dellaporta>Maize mini prep and
Rogers/Bendich >CTAB method).
I`m searching for a genomic DNA isolation method from calli.
Any help would be appreciated.

Maren Jacob
maren at rhrk.uni-kl.de 



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