Protein purification course offered by Cook College of Rutgers-Univ.

Minnam Sivananda minnam at yoko.rutgers.edu
Mon Feb 15 14:58:49 EST 1993


		Rutgers-The State University of New Jersey
		Campus at New Brunswick

	Continuing Professional Education Programs in Biotechnology

The Cook College of Rutgers- The State University of New Jersey is offering a hands-on Laboratory course in the field of Biotechnology.


Protein Purification: Isolation, Analysis and Characterization

Three Offerings:
	March 14-19,1993
	June 13-18,1993
	July 18-23,1993

Course description
	This 5- 1/2 day laboratory course covers a wide varietyof conventional methods for protein isolation, purification and characterization. Th course format integrates hands-on laboratory exercises with class room lectures, demonstrations, study breaks and short take-home assignments.
	A special feature of the course is that all laboratory work will be performed  on the same starting sample which will be purified from an exceedingly crude form to near homogenity as judged by HPLC, SDS gel electrophoresis and isoelectric focusing. This feature provides a continuity of purpose , integrating dozens of preparative and analytical protein techniques in a way that few competing courses can match.
	A problem solving approach will be used throughout the course. Under the guidance of experienced lab instructors, participants will work in groups of three to plan their own protocols, analyze data and interpret results. A student teacher ratio no greater than 8:1 will be maintained and faculty co-ordinators will be present throughout the course.

Techniques and Instruments you will use:
* Tissue Homogenizers * Filtration devices including  Tangential Flow Ultrafiltration * Refrigerated (Sorvall) centrifuges * Recording UV-Vis Spectrophotometers * Filter Fluorometers * Luminescence photometers * Column Chromatography (gel filtration , Ion exchange and Hydrophobic Interaction) * Fraction - Collectors * Column Monitors * Electrophoresis (SDS,native and isoelectric focusing) * Coomassie Blue and Silver Staining * Reverse Phase HPLC * FPLC and Phast System * Amino Acid analysis * Automated Edm



an sequencing * DNA Sequence Analysis * Peptide Mapping * Mass Spectral Analysis 

For more information :

Call Pam  at (908)932-9271
or 
Send mail to : Cook College
		Office of Continuing Professional Education
		P.O Box 231
		New Brunswick NJ-08903-0231
or
Send email to : czesak at gandalf.rutgers.edu
or 
Send Fax to : (908)932-8726
		
  


 



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