Rutgers-The State University of New Jersey
Campus at New Brunswick
Continuing Professional Education Programs in Biotechnology
The Cook College of Rutgers- The State University of New Jersey is offering a hands-on Laboratory course in the field of Biotechnology.
Protein Purification: Isolation, Analysis and Characterization
This 5- 1/2 day laboratory course covers a wide varietyof conventional methods for protein isolation, purification and characterization. Th course format integrates hands-on laboratory exercises with class room lectures, demonstrations, study breaks and short take-home assignments.
A special feature of the course is that all laboratory work will be performed on the same starting sample which will be purified from an exceedingly crude form to near homogenity as judged by HPLC, SDS gel electrophoresis and isoelectric focusing. This feature provides a continuity of purpose , integrating dozens of preparative and analytical protein techniques in a way that few competing courses can match.
A problem solving approach will be used throughout the course. Under the guidance of experienced lab instructors, participants will work in groups of three to plan their own protocols, analyze data and interpret results. A student teacher ratio no greater than 8:1 will be maintained and faculty co-ordinators will be present throughout the course.
Techniques and Instruments you will use:
* Tissue Homogenizers * Filtration devices including Tangential Flow Ultrafiltration * Refrigerated (Sorvall) centrifuges * Recording UV-Vis Spectrophotometers * Filter Fluorometers * Luminescence photometers * Column Chromatography (gel filtration , Ion exchange and Hydrophobic Interaction) * Fraction - Collectors * Column Monitors * Electrophoresis (SDS,native and isoelectric focusing) * Coomassie Blue and Silver Staining * Reverse Phase HPLC * FPLC and Phast System * Amino Acid analysis * Automated Edm
an sequencing * DNA Sequence Analysis * Peptide Mapping * Mass Spectral Analysis
For more information :
Call Pam at (908)932-9271
Send mail to : Cook College
Office of Continuing Professional Education
P.O Box 231
New Brunswick NJ-08903-0231
Send email to : czesak at gandalf.rutgers.edu
Send Fax to : (908)932-8726