I am looking for an antibody (rabbit) to use as a loading control for a western
blot. I need an antibody that will bind to a protein in a crude extract from
whole Arabidopsis plants and will give me a single band that I can use to
compare different lanes within a gel to ensure that they are equally loaded.
I detect the primary antibody with a goat-anti-rabbit IgG fused to HRP. Does
anyone out there have such an antibody which they would be willing to send
me, or does anyone know of a commercial source? Thank you.
Joel A. Kreps
Dept of Bioch. and Molec. Biology
U. Mass at Amherst