cloning RAPDs

scolnipa at scolnipa at
Mon Jan 18 17:54:31 EST 1993

>We use the InVitrogen kit, and have found that "white" colonies very rarely
>contain the insert of interest!  However, the "light blue" (not dark blue)
>colonies usually do!?!?  But we had to look through a number of these as 

Same experience here.  It helps to omit the IPTG.

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