Quantitative PCR for RNA analysis and CaMV promoter

ELIZ at CCIT.ARIZONA.EDU ELIZ at CCIT.ARIZONA.EDU
Wed Jan 20 15:50:05 EST 1993


Dear Networkers,
	Can anyone give me information about protocols and pitfalls for
performing PCR to detect mRNA in small tissue samples of Arabidopsis?
Are there references which describe using this with Arab for qualitative
or quantitative purposes.  Does anyone have a protocal that works, on
the gopher or elsewhere?  Seems like the right technique for a small
organism.
	My second question in with regard to expression of the
35S promoter in developing Arabidopsis seeds.  Is this a strong
promoter in late seed maturation?  Is it active throughout the seed?
Any specific references I should consult?
	Thanks for any info.  ELIZABETH VIERLING. ELIZ at ccit.arizona.edu



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