transient expression in protoplasts

GBCA59 at vms1.glasgow.ac.uk GBCA59 at vms1.glasgow.ac.uk
Sun Jul 25 11:12:00 EST 1993


Hello from sunny Glasgow,
Has anyone got a good working protocol for transient expression in Arabidopsis
protoplasts. We have made mesophyll protoplasts and electroporated 35S/GUS
and 35S/CAT into these using an IBI Gene Zapper450/2500. We got 50% survival
after 24 hours but no reporter gene activity. Ultimately we want to use
cell cultures and chalcone synthase/reporter gene constructs. Any ideas or
references would be very useful since we have had no luck in searching the
literature for methods.
Thank you for your time and help
Ian Graham/ John Christie
Dept. Biochemistry, University Glasgow
Glasgow G12 8QQ,  United Kingdom



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