positive control antibody for in situ work

markley at ACS.UCALGARY.CA markley at ACS.UCALGARY.CA
Thu Jun 10 18:49:25 EST 1993


I am conducting in situ experiments with polyclonal 
antibodies raised to the Proliferating Cell Nuclear 
Antigen (PCNA) from Brassica napus.In other eukaryotes,
PCNA has been shown to be located in the nucleus where 
it performs a function in DNA replication. I have encountered
difficulty detecting the antigen in tissue sections fixed 
under a variety of conditions and embedded in different 
media. In order to address the problem at hand I require 
an antibody for use as a positive control.More specifically, 
I require antibodies (monoclonal or polyclonal) directed 
to plant histones or any plant nuclear protein. If anyone 
has such an antibody and is willing to part with a small 
sample please contact me. I would greatly appreciate 
the help. 
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

   Nancy A. Markley		 *     Telephone : (403) 220-7907
   Dept.Biological Sciences	 *           Fax : (403) 289-9311
   University of Calgary	 *	  E-mail : markley at acs.ucalgary.ca
   2500 University Dr. NW	 *		   
   Calgary, Alberta,		 *
   Canada. T2N-1N4		 *

!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!



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