Help for T-DNA borders

Jim Haseloff jph at MRC-LMB.cam.ac.uk
Wed Jun 30 07:36:00 EST 1993


Hi Tiesheng Gu -
Maybe 20 other people have already mailed you this - oh well, this is the
region around the right border of pTiT37 - and which is also in the pBIN
derivatives. In pBIN19, its followed by the KanR gene, rather than nopaline
synthase gene in pTiT37. You should follow the manipulations outlined in
Mike Bevan's original paper (describing pBIN19) to get the full story, NAR
12:8711 (1984)

                      AACCCTGTGG TTGGCATGCA CATACAAATG
GACGAACGGA TAAACCTTTT CACGCCCTTT TAAATATCCG TTATTCTAAT                     
                   
AAACGCTCTT TTCTCTTAGG TTTACCCGCC AATATATCCT GTCAAACACT
                    ^ ^^^^^^^^^^ ^^^^^^^^^^ ^^^^ 25 base repeat
GATAGTTTAA ACTGAAGGCG GGAAACGACA ATCTGATCAT GAGCGGAGAA
TTAAGGGAGT CACGTTATGA CCCCCGCCGA TGACGCGGGA CAAGCCGTTT
TACGTTTGGA ACTGACAGAA CCGCAACGAT TGAAGGAGCC ACTCAG(CCGCGG) - SacII site

This sequence was derived from:
>>>>ATNOPA 8/8
Agrobacterium tumef. gene encoding nopaline synthetase. (Resident in
plasmid pTiT37.). 8/87
ID   ATNOPA     standard; DNA; 2521 BP.
AC   V00087;
DT   03-AUG-1987 (an correction)
DT   03-MAR-1983 (no comment)
DE   Agrobacterium tumef. gene encoding nopaline synthetase.
DE   (Resident in plasmid pTiT37.)
KW   nopaline synthetase; plasmid; synthetase.
OS   Agrobacterium tumefaciens
OC   Prokaryota; Bacteria; Gram-negative aerobic rods and cocci;
OC   Rhizobiaceae.
RN   [1] (bases 183-2521)
RA   Bevan M., Barnes W.M., Chilton M.D.;
RT   "Structure and transcription of the nopaline synthase gene region
RT   of T-DNA";
RL   Nucleic Acids Res. 11:369-385(1983).
RN   [2] (bases 1-2521)
RA   Depicker A., Stachel S., Dhaese P., Zambryski P., Goodman H.M.;
RT   "Nopaline synthase: Transcript mapping and DNA sequence";
RL   J. Mol. Appl. Genet. 1:561-573(1982).
CC   Sequence taken from reference [2].
FH   Key        From     To       Description
FH
FT   CONFLICT    190    191       GT is GAT in [1]
FT   CONFLICT    378    380       GAT is GT in [1]
FT   MSG         550   2002       messenger RNA
FT   CDS         585   1823       coding sequence
FT   CONFLICT   1249   1249       G is A in [1]
FT   CONFLICT   1661   1661       C is A in [1]
FT   CONFLICT   1668   1668       C is A in [1]
SQ   Sequence  2521 BP;  681 A; 622 C; 556 G; 662 T; 0 other;
.



More information about the Arab-gen mailing list