plaque hybridization

fzbritt at HAMLET.UCDAVIS.EDU fzbritt at HAMLET.UCDAVIS.EDU
Thu Nov 4 12:40:42 EST 1993


A technical question- we're probing a genomic library in lambda with
an Arabidopsis cDNA.  Can I cram a bunch of those large plaque lift
membranes into a single 2-inch hybridization tube (used with a rotisserie
oven)?  It doesn't seem possible that the hybridization mix can coat all
that tightly rolled surface.  If anyone has had a positive experience
with this particular technique, let me know.
thanks,
Anne Britt



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