histone H4 expression

unknown at dl.ac.uk unknown at dl.ac.uk
Thu Nov 18 06:22:28 EST 1993

We have been using a histone H4 genomic fragment from Ben Scheres,
Netherlands as a control on Northerns for cell-cycle expression. We see
three RNA bands of about 1.9kb, 2.9kb and 8.0kb. The probe was a 0.7kn
BamHI-HindIII fragment on total RNA.
I expected to see a single RNA species. Has anyone else seen this or is it
background? All three bands appear to be regulated together.
Thanks for any help

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Jim Murray
Institute of Biotechnology
University of Cambridge
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