Yac Request, m249

John Morris MorrisJohn at FRODO.MGH.HARVARD.EDU
Tue Nov 23 09:49:41 EST 1993


>Dear Networkers,  has anyone already mapped marker m249 (chrs 3) to a YAC? If s
>o are they prepared to share the information as to which YAC(s) is/are relevent
>?  Please reply either to the network, or K445711 at czhrzu1a    (bitnet address)
> Thank you in advance,   Alan Slusarenko

Alan,
If you haven't looked, the AAtDB gopher server contains several entries on
m249 that may be of interest.  In addition to the information that I've
appended, there a several entries in the database on the two-point mapping
data.  However, I don't see any YAC hybridizations.  

John Morris
Curator, 
MGH Arabidopsis Database Project

Strain : "CD1-42"
  Ordering_info   Order_strain_from   Ohio_State
  Features    Locus   m249
              Probe   m249
  Type    Cloned_DNA
  Image   meyerowitz-lambda-chr3

Probe : "m249"
  Strain      CD1-42
  Locus   m249
  RFLP_mapping    Meyerowitz lab      EcoRI   Nied_x_Col      polymorphic
                                      SacI    Nied_x_Col      polymorphic
                                      XbaI    Nied_x_Col      polymorphic
                                      XhoI    Nied_x_Col
                                                             
dominant/recessive 
                                                                (extra band(s))
                                              Col_x_Land
                                                             
dominant/recessive 
                                                                (extra band(s))
                                      BglII   Col_x_Land      polymorphic
                                              Nied_x_Land     polymorphic
                  Goodman lab     HindIII     Col_x_Land      polymorphic but 
                                                                not used
                                  BclI    Col_x_Land      polymorphic but not 
                                                            used
                                  EcoRI   Col_x_Land      polymorphic but not 
                                                            used
                                  ClaI    Col_x_Land      polymorphic but not 
                                                            used
                                                          used for map
                                  DraI    Col_x_Land      polymorphic but not 
                                                            used
                                  BglII   Col_x_Land      polymorphic but not 
                                                            used
                                  XbaI    Col_x_Land      not polymorphic
                  Data    Mapped_bands    1L 2L 3C 4C 5C ClaI
                  Autorad     m249
Locus : "m249"
  Type    RFLP
  Name    Probe_desc      random lambda clone
  Molecular_information   Probe   m249
  Image   m249
  Strain      CD1-42
  Map     Genetic     gMap    Unified-III     66.7    1.48
                              Visible-III     73.6    1.04
                              1.0_Integrated-III      65.4    0.28
                              Scolnik_All-III     59.5    0.68
          Position_ambiguous      See Reiter et al PNAS 89:1477; approx. 3.5 cM 
                                    from r268
          Mapping_data    F2_data     Goodman_3
                                      Meyerowitz_3
                          RI_data     Scolnik_3
                                      Dean_RI_3
                          2Point      g4708-m249-1398
                                      GS-KB6-m249-1411
                                      g6220-m249-1421
                                      abi3-m249-1430
                                      BWS12-m249-1441
                                      m433-m249-1450
                                      g17287-m249-1459
                                      GAP-A-m249-1467
                                      g2440-m249-1476
                                      gl1-m249-1482
                                      pAt3-89.1-m249-1487
                                      PB3-m249-1498
                                      m249-g4117-1509
                                      m249-g2534-1510
                                      m249-g4564-b-1511
                                      m249-g4014-1512
                                      m249-g17811-1513
                                      m249-g4125-1514
                                      m249-g2606-1515
                                      m249-tt5-1516
                                      m249-g2778-1517
                                      m249-XDB-G-1518
                                      m249-g19826-1519
                                      m249-g5966-1520
                                      dwf1-m249-2289
                                      m105-m249-2298
                                      m255-m249-2308
                                      m433-m249-2310
                                      KG-23-m249-2316
                                      g2440-m249-2320
                                      gl1-m249-2324
                                      m281b-m249-2330
                                      m249-CAB11-2335
                                      m249-csr-2336
                                      m249-m409-2337
                                      m249-m576-2338
                                      m249-apZL-2339
                                      m249-m457-2340
                                      m249-ap3-2341
                                      m249-g4014-2342
                                      m249-Syac1G12-2343
                                      m249-m115-2344
                                      m249-m339-2345
                                      m249-u2r3-2346
                                      m249-pCIT1210-2347
                                      m249-m460-2348
                                      m249-m424-2349
                                      axr2-m249-262
 





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