PCR - multiple bands

Ashleigh Mckenzie MCKENZIEA at za.ac.unp.cc.gate2
Fri Oct 8 08:21:22 EST 1993


I have trouble getting specificity when amplifying lambda gt11
inserts. Even after isolating the desired single band from a low
melting point gel, and PCR'ing this, the second PCR resolves into
multiple bands again. Could this have anything to do with
secondary structure? Any comments or advice to solve this problem
would be appreciated.

Ashleigh

E-mail address: mckenziea at botany.unp.ac.za



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