PCR - multiple bands
Robert F. Whittier
101002.2366 at CompuServe.COM
Fri Oct 8 23:30:36 EST 1993
Dear Ashleigh & networkers,
To resolve the problem of single isolated PCR bands regenerating into
multiple bands, the simplest obvious solutions are to try higher
annealing temperatures and/or use a "hot start" protocol in your re-
amplification reaction. But your primers may be the real culprits.
For an enlightening discussion of this, see the recent one page
article by Crameri & Stemmer, NAR 21, 4410.
Mitsui Plant Biotechnology Research Institute
TCI A10, Sengen 2-1-6
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