PCR from solid tissue ?

Nadine Smith nmsmith at sunflower.bio.indiana.edu
Mon Oct 11 12:06:54 EST 1993


In article <9310110929.AA25040 at net.bio.net> vachon at BIO.GRENET.FR (Gilles VACHON) writes:
>
>Hi,
>Having problems to collect large amounts of material, I would like to know
>if some of you have heard about PCR directly from very small amounts of
>plant material, (e.g. ONE meristem...)
>Also, if possible, can we imagine doing a cDNA bank from a piece of tissue
>that small after RT and PCR amplification?  
>
>Is there a PCR God on the list who can answer these questions?
>Thanks in advance.
> 
>Gilles Vachon
>University of Grenoble
>France
>Tel: (33) 76 51 45 48 
>Fax: (33) 76 51 43 36
>e-mail: vachon at bio.grenet.fr
>

I have used the alkali treatment prep from Klimyuk, et al. (Plant Journal,
vol. 3, pp. 493-494, 1993) with great success.

Nadine Smith
Indiana University, Biology--Plant Sciences
nmsmith at bio.indiana.edu

"Listen to the flowers, they will tell you sweet secrets" 



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