cDNA library from dark grown tissue *** forwarded ***

S. Marshall S.Marshall at
Wed Oct 27 04:16:44 EST 1993

	From hofte at fr.inra.versailles.tournesol Tue Oct 26 08:13:16 1993
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	From: Hermann Hofte <hofte at fr.inra.versailles.tournesol>
	Original-Sender: Hermann Hofte <hofte at fr.inra.versailles.tournesol>
	Reply-To: Hermann Hofte <hofte at fr.inra.versailles.tournesol>
	Subject: Re: cDNA library from dark grown tissue
	To: jkreps at edu.umass.biochem
	Cc: "bionet.genome.arabidopsis mail newsgroup" <bionet-news at>
	In-Reply-To: <931025185134.~INN-EAAa19100.bionet-news at>
	Message-Id: <Pine.3.07.9310260943.A19310-b100000 at tournesol>
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	On 25 Oct 1993 jkreps at wrote:
	> Dear Network,
	> I am interested in isolating a cDNA clone for a sequence related to 
	> a gene I have been studying.  I observe this sequence in northern
	> blots of RNA from dark-grown Arabidopsis.  Before I construct a
	> cDNA library of my own, I thought I would check to see if any one
	> has a such a library and if he or she would be willing to send it
	> to me.  The sequence of interest is between 1000 and 1500 b in size and
	> is observed maximally within 24 hours of putting light-grown plants
	> into dark conditions.  Thanks in advance.
	Dear Joel Kreps,
	We made a cDNA library from etiolated Arabidopsis seedlings. It is being
	used for our systematic cDNA sequencing project. The library was
	constructed in a plasmid after size fractionation of the cDNAs into three
	size classes (0.5-1kb, 1-2.2kb, >2.2kb). I could send you a lyophilized
	miniprep of the amplified library corresponding to the second sizeclass>
	Please let me know your postal address if you would like to receive a sample..
	Herman Hofte

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