Bacterial artifical chromosomes and P1

PN18000 PN18 at MUSICA.MCGILL.CA
Sat Sep 18 09:54:14 EST 1993


We have identified markers that are tightly linked to a viral
resistance gene.  We must obtain large DNA clones, and would rather
avoid YACs.  Anyone has any feedback on Bacterial Artificial Chromosomes
?
At what rate are clones obtained compared to YACs?  Their average size?
What vector do you recommend?
How about P1 clones?  They do not seem to have been used much?
Why not? (besides their size).

I'll summarize answers if I get enough response.

Marc Fortin
Plant Science
McGill University
pn18 at musica.mcgill.ca



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