T-DNA sequence/plasmid rescue

[TAGUE at FRODO.MGH.HARVARD.EDU]

Greetings netters:

Three different yet related questions:

1) Has the sequence of the Feldmann T-DNA (3850:1003) been completed? Is the 
sequence available?

2) What restriction enzymes have folks used (besides EcoRI and SalI) for
successful plasmid rescue?

3) Have folks sequenced rescued plasmids with T-DNA-based primers? Are there
sequences with reasonably good chances of being included in a rescued
plasmid?

Thanks much!

Brian W. Tague
Mass. General Hospital


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