Inducible Arab. promoters and Protein:: Gus fusions

John Hays haysj at BCC.ORST.EDU
Tue Sep 28 20:11:02 EST 1993


I am trying to develop a new technique for screening for Arabidopsis
mutants defective in UV repair.  Without going into details, I am
soliciting ideas, and references for two critical elements.
	1. A highly inducible but tightly controlled Arabidopsis promoter
(or plant promoter that functions in Arabidopsis, with the following
properties):
	a. Very low constitutive expression.
	b. Rapidly induced, at least 10-20x, by a convenient-to-use signal.
	c. Ideally, the signal should not be likely to itself cause DNA
damage, e.g. UV, or affect other plant activities, e.g. heat shock.
	2. A gene whose product, when fused translationally to (3 prime)
GUS, does not interfere with Beta-glucuronidase expression.  Ideally the
gene (including its introns), should span at least 10 kb of DNA.
	Any suggestions would be greatly appreciated.





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