CAP marker GAP B
acernac at bio.indiana.edu
Tue Apr 26 09:45:55 EST 1994
At the moment I'm stuck in the abyss of PCR Hell. I would appreciate
any advice anyone can give me on getting amplification of CAP marker GAP B
(Konieczny and Ausubel, The Plant Journal, 1993, 4(2) 403-415). I have
changed my solutions and altered MgCl levels, as well as trying various DNA
samples. I am trying to amplify from genomic DNA from Colombia and
Landsberg. I have also tried other CAP primers and I haven't gotten
amplification from those either. The conditions are the same as stated in
the research article. Any advice would be helpful.
A second question: Does anyone know of any enzymes that will get a
RFLP between NDO and Col using CAP marker GAP B PCR product?
Thanks for any suggestions,
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