On 5 Aug 1994, jinsong sheng wrote:
Any suggestions to separate a 65 and 64 kd proteins on SDS -PAGE? Like
percentage of gels, gradient or buffer systems?
Dear Jinsong Sheng,
Refer to these papers for separation of proteins,
1) Tricine-SDS polyacrylamide gel electrophoresis for the separation of
proteins in the range from 1 to 100 K. Anal. Biochem. 166, 368-379.
2) Architecture of physalis mottle tymovirus as probed by monoclonal
antibodies and cross-linking studies. Virology, 193, 959-966. In this
article authors show separation of 15 and 16 K proteins; 5.4 and 4.7K
S.P. Dinesh-Kumar (Dr. Barbara Baker's lab)
Plant Gene Expression Center
Albany, CA 94710