DNA methylation
Carolyn M Wetzel
cmwetzel at iastate.edu
Fri Dec 2 13:29:28 EST 1994
Hello,
I am analyzing methylation of a specific DNA region in an
Arabidopsis mutant. So far I have tried HpaII versus MspI (CCGG versus
C5mCGG) but would like to use an enzyme that cuts 5mCCGG and one that
cuts regardless of methylation at both sites (MspI will cut only if the
outer C is unmethylated). Is this just wishful thinking, or do these
enzymes really exist? I've looked through some catalogs and papers, but
with no luck.
Any suggestions or pointers to references will be useful.
Thank you, Carolyn
--
Carolyn M. Wetzel cmwetzel at iastate.edu
Postdoctoral Research Associate Tel. 515/294-7784
Department of Botany Bessey Hall
Iowa State University Ames, Iowa 50011-1020
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