Arabidopsis Course

Robert Last rll3 at CORNELL.EDU
Fri Dec 2 15:11:30 EST 1994


Dear Netters,
Below is a description of the Cold Spring Harbor 'Arabidopsis' Course, to
be held in July, 1995. Application and information can be obtained from:

Meetings Office
Cold Spring Harbor Lab
1 Bungtown Road
Cold Spring Harbor, NY 11724

A couple of noteworthy points:
-although the course has Arabidopsis as the main focus, the lectures will
include a number of speakers who will describe work done in other model
plant species including brassica, chlamydomonas, maize, tomato etc.
-we expect to be able to provide some partial scholarships for
students/postdocs.

Please feel free to contact any of the organizers for further information.

For the organizers,
Rob Last
____________________________


ARABIDOPSIS MOLECULAR GENETICS
July 3 - 23, 1995

Xing-Wang Deng, Yale University
Robert Last, Boyce Thompson Institute at Cornell University
Daphne Preuss, University of Chicago

This course provides an intensive overview of topics in plant growth and
development, focusing on molecular genetic approaches to understanding
plant biology.  It emphasizes recent results from Arabidopsis thaliana and
other model plants and provides an introduction to current methods used in
Arabidopsis research.  The course also demonstrates the use of microbial
systems in plant research, including Agrobacterium, E. coli, and S.
cerevisiae.  It is designed for scientists with experience in molecular
techniques or in plant biology who wish to work with Arabidopsis.  The
course consists of  a vigorous lecture series, a hands-on laboratory, and
informal discussions.  Speakers will provide both an in-depth discussion of
their own work and a review of their specialty.

Discussions of important topics in plant research will be presented by the
instructors and by invited speakers.  These seminars will review plant
anatomy; plant development (including development of flowers, roots,
meristems, embryos and the epidermis); perception of light and
photomorphogenesis; responses to pathogens and to DNA damage; synthesis and
function of secondary metabolites and hormones; nitrogen assimilation;
unique aspects of plant cell biology (including the plant cytoskeleton,
cell wall, and chloroplasts); the importance of transposons and
Agrobacterium for manipulating plant genomes and current approaches to
genome analysis.

Invited speakers include: J. Bender, A. Britt, C. Chapple, J. Chory, G.
Drews, J. Ecker, S.Y. He, A. Lloyd, H. Ma, R. Martienssen, J. Medford, T.
Mitchell-Olds, P. Quail, J. Schiefelbein, B. Staskawicz, D. Stern, I.
Sussex, T. Voelker, and V. Walbot.

The laboratory sessions will provide an introduction to important
techniques currently used in Arabidopsis research.  These include studies
of Arabidopsis development, mutant analysis, studies of epidermal features,
in situ detection of RNA, histochemical staining and immunolabeling of
proteins, transformation with Agrobacterium, transient gene expression in
protoplasts, expression of plant proteins in microorganisms, detection and
analysis of plant pathogens, and techniques commonly used in genetic and
physical mapping.






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