Epitope TAG and trichome section

Koji Goto kgoto at SCL.KYOTO-U.AC.JP
Mon Dec 19 07:34:04 EST 1994


Dear netters,

1)
        Does anyone use or know good combinations of epitope TAG and its
antibody which can use for immunohistochemistry?  Some commercial antiboies
are good for immunoprecipitation, but too weak to detect in wax section.
        
2)
       We are trying to make sections of trichome for in situ
hybridization.  In wax embedded samples, most trichomes are broken.  In
Technovit resin, trichomes are kept their shapes but hard to perform in
situ hybridization.  
        Is there any trick to make good section of wax embedded trichome? 

Any experiences or suggestions are welcome.
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
Koji GOTO
>>New Address<<
Molecular Biology and Information (II)
Institute for Chemical Research
Kyoto University
Uji, Kyoto, 611, Japan
Phone: +81-774-32-3111 (x2208)
FAX/Voice: +81-774-32-8336
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