GAPA CAPS problems

century at MENDEL.BERKELEY.EDU century at MENDEL.BERKELEY.EDU
Tue Feb 15 18:24:50 EST 1994


Hi Netters,

I have been using the CAPS markers and have been getting pretty good
results with the ones that I've tested except for GAPA.  I cannot seem to
get good, reproducible amplification using the same conditions (essentially
as described in the Konieczny and Ausubel Plant Journal paper) that work
for the other primers.  I've had the primers resynthesized, and still have
not had much success.  Has anyone else had problems with this pair of
primers?  Any suggestions and/or possible solutions?

Thanks,
Karen Century
century at mendel.berkeley.edu




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