reply to NAASC letter

Joe Ecker jecker at ATGENOME.BIO.UPENN.EDU
Fri Jun 3 14:12:26 EST 1994


RESPONSE # 12


1. Do you think that we are ready to begin some level of directed genome 
sequencing in the US?  YES, IMMEDIATELY.  

2. How important is genome sequencing in terms of funding priorities (vs. 
placing cDNAs on the map, completion of the physical map, adding more 
PCR-based markers to the map, etc.)?  SOME MONEY SHOULD BE ALLOCATED TO SEVERAL CENTERS AT ONCE.

3. Who should support systematic genome sequencing if it is a big-$ effort?  PROBABLY THE USDA.  SAVE NSF MONEY FOR "SMALL SCIENCE".

4. What impact on Arabidopsis research will be incurred if sequencing does not
begin today (in 2 years; in 5 years,  in 10 years)?  NEGATIVE AND SEVERE.  A GENOME EFFORT PROVIDES A WAY TO ELEVATE ARABIDOPSIS TO WORLD-CLASS STATUS AND SHOULD BE STARTED NOW.

5. What type of organizational model for genome sequencing would you support: 
sequencing centers vs. individual interested labs?  DEFINATELY CENTERS. INDIVIDUAL LABS COULD THEN CARRY OUT PHYSICAL MAPPING AND OTHER TASKS. 
                        
6. What quality standards would you expect for the sequence: high or low  
accuracy (high accuracy = higher cost)?  HIGH.  A 5-10 FOLD REDUNDANCY IS PROBABLY INEVITABLE ANYWAY.
                                                                          
7. ANY SPECIFIC OR GENERAL COMMENTS THAT YOU WOULD LIKE TO MAKE!  PLEASE DO NOT CONSIDER SEQUENCING THE GENOMES OF OTHER PLANTS.  AT 100 MEGABASES, ARABIDOPSIS WILL BE PLENTY DIFFICULT!



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