promoters (fwd)

Kevin Farnden kevinjff at SANGER.OTAGO.AC.NZ
Thu Jun 16 16:01:29 EST 1994



On Thu, 16 Jun 1994, Neil Butt wrote:

> Forwarded message:
> > From root Thu Jun 16 10:37:53 1994
> > Via: arcs.afrc.ac.uk; Thu, 16 Jun 1994 10:40:36 +0100
> > Via: uk.ac.sussex; Thu, 16 Jun 1994 10:38:03 +0100
> > Message-Id: <22473.199406160935 at solx1.central.susx.ac.uk>
> > From: Neil Butt <N.J.Butt at sussex.ac.uk>
> > Subject: promoters
> > To: arabuk at bbsrc.ac.uk
> > Date: Thu, 16 Jun 1994 10:35:30 +0100 (BST)
> > X-Mailer: ELM [version 2.4 PL23]
> > Content-Type: text
> > Content-Length: 974
> > Sender: arabuk-request at afrc.ac.uk
> > 
> > Dear community
> > 
> > I am trying to find an inducible promoter to construct a vector in
> > which we can regulate expression. The promoter we require needs to be
> > active in tobacco and quite tightly regulated. I have obtained steriod
> > inducible promoters (Davis et al ), but these only work in suspension
>  cultures because the steroid stimulus is difficult to present to whole
>  plants. I have also looked at the paper by Hershey and Stone which
>  describes a promoter which is induced by herbicides. This group work for
>  DuPont and i am not sure if this will present difficulties in obtaining
>  the clones.
>  
> > What I wish to know is, does any one have such a vector? If not does any
> > one know how I can get hold of a promoter to maybe construct such a
> > vector. The protein we wish to observe may be lethal to plants if
> > expressed at too high a level, so to obtain transgenics we need this
> > sort of expression system.
> > 
> > Hope some one can help
> > 
> > Neil Butt
> > Dept Biochemistry
> > University of Sussex
> > Brighton, BN1 9QG
> > U.K
> > 
> 
> 
Try the copper inducible system developed by  Paul Reynolds. The original
reference is PNAS 90 4567-4571. The system has been improved so contact
Paul for the latest developments. His email is preynolds at hort.cri.nz
Kevin Farnden
Dept Biochemistry
Uiversity of Otago
Dunedin
New Zealand 





More information about the Arab-gen mailing list