promoters (fwd)

Neil Butt N.J.Butt at sussex.ac.uk
Thu Jun 16 04:47:37 EST 1994


Forwarded message:
> From root Thu Jun 16 10:37:53 1994
> Via: arcs.afrc.ac.uk; Thu, 16 Jun 1994 10:40:36 +0100
> Via: uk.ac.sussex; Thu, 16 Jun 1994 10:38:03 +0100
> Message-Id: <22473.199406160935 at solx1.central.susx.ac.uk>
> From: Neil Butt <N.J.Butt at sussex.ac.uk>
> Subject: promoters
> To: arabuk at bbsrc.ac.uk
> Date: Thu, 16 Jun 1994 10:35:30 +0100 (BST)
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> 
> Dear community
> 
> I am trying to find an inducible promoter to construct a vector in
> which we can regulate expression. The promoter we require needs to be
> active in tobacco and quite tightly regulated. I have obtained steriod
> inducible promoters (Davis et al ), but these only work in suspension
 cultures because the steroid stimulus is difficult to present to whole
 plants. I have also looked at the paper by Hershey and Stone which
 describes a promoter which is induced by herbicides. This group work for
 DuPont and i am not sure if this will present difficulties in obtaining
 the clones.
 
> What I wish to know is, does any one have such a vector? If not does any
> one know how I can get hold of a promoter to maybe construct such a
> vector. The protein we wish to observe may be lethal to plants if
> expressed at too high a level, so to obtain transgenics we need this
> sort of expression system.
> 
> Hope some one can help
> 
> Neil Butt
> Dept Biochemistry
> University of Sussex
> Brighton, BN1 9QG
> U.K
> 




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