daylength for transformants
talbert at U.WASHINGTON.EDU
Wed Mar 9 15:59:34 EST 1994
Thanks to all who replied to my query about light regimes for
tranformations. Rick Amasino, Yi-Fang Tsay, Anna Astromoff, George
Karlin-Neumann and Mary Honma all reported success with continuous light.
Mary Honma reported comparable results with either continuous light or 16
hr days, but stressed the desirability of keeping the temperature at 22C.
In addition, a co-worker drew my attention to the report of Chaudhury and
Signer (Plant Cell Reports 8: 368-369) showing better regeneration under
low light (20 microE/sec/m2) for cotyledons.
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