DNA preparation of low copy cosmids

Nancy Terryn nater at gengenp.rug.ac.be
Sat May 21 04:41:12 EST 1994


Dear netters,

we are trying to prepare large amounts of DNA from some low copy cosmids 
based on pRK290 using a normal alkali lysis protocol. However we get 
very little DNA out of it. Does anybody have some suggestions on how we 
could improve our yield? The DNA we need has to be very pure (no 
chromosomal DNA contamination). I would really appreciate if I would get 
some suggestions!

Thank you all!

Nancy Terryn
Lab of Genetics
K.L. Ledegankcstraat 35
9000 Gent/ Belgium

Tel 32 9 264 50 10
Fax 32 9 264 53 49






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