isolation of nuclei from arabidopsis

gaudino at WUSTLB.WUSTL.EDU gaudino at WUSTLB.WUSTL.EDU
Thu May 26 17:20:09 EST 1994


Netters, 
I have recieved a protocol for the isolation of nuclei for nuclear run-ons 
from Dr. Elaine M. Tobin.  The reference she sent me via e-mail was 
Brusslan et al, The Plant Cell(1993) vol 5 pgs 667-677.  From a quick 
glance the procedure seems to be simplicity itself.  This reference also 
gives a previous Tobin lab reference for invitro transcription.  This 
second reference is Silverthorne and Tobin (1984) PNAS vol 81, pgs 1112-
1116.  I hope this information helps everyone as much as it will help me.  
Furthermore, I would like to thank Dr. Tobin for taking time to respond to 
my inquery as promptly as she did.  
					Reggie Gaudino

................................................................................
Reggie Gaudino			Internet:     gaudino at wustlb.wustl.edu
				BITnet:       GAUDINO at WUSTLB
Washington University		Biology Department  Box 1137
St. Louis, Missouri  63130	(314)935-8529



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