5' RACE PCR tailing

Russell L. Malmberg russell at dogwood.botany.uga.edu
Mon Sep 5 09:27:37 EST 1994


 We need to do some 5' RACE (or anchored) PCR with
 Arabidopsis cDNAs.  In some protocols G or C tailing
 is used, in others, T or A tailing is used because of
 the lower Tm.

 We wondered if the relatively high AT content of
 Arabidopsis might make it a bad idea to use T or A 
 tailing of 5' ends of cDNAs.  Could the high internal
 AT content cause spurious PCR priming?

 Does anyone have suggestions about this?

 Thanks,

 Russell

================================
 Russell L. Malmberg
 russell at dogwood.botany.uga.edu
================================



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