Plants making plastic ?
jwm at thale.mgh.harvard.edu
Wed Apr 19 16:05:29 EST 1995
cal_lab at biosci.uq.oz.au (hiv_emir) wrote:
> I'm trying to hunt down some reference on polyhydroxybutyrate
> production by arabidopsis. There was a recent article in
> new Scientists referring to PNAS, but only in passing.
> The Jan/Feb issues are still not is OZ yet ? Does anyone
> have the actual page nos and authors etc ?
> I'm trying to develop a problem-based learning exercise
> on this unusual aspect of "plant metabolism".
> Craig Zimitat
Using the WAIS indexed files of AAtDB on gopher, and the query word
polyhydrox*, I find the reference below. The gopher server is
available at <gopher://weeds.mgh.harvard.edu>.
If you do not have a gopher client, but do have telnet access,
you can telnet to weeds.mgh.harvard.edu and use the login name gopher.
- John Morris
Paper : "poiri-1992-aafzj"
Reference Title Polyhydroxybutyrate, a biodegradable thermoplastic,
produced in transgenic plants
Journal Science (Washington DC)
Agricola_ID IND92045876. 9209.
Page 520 523
Author Poirier, Y.
Dennis, D. E.
Type Journal Article
Polyhydroxybutyrate (PHB), a high molecular weight
polyester, is accumulated as a storage carbon in many species of
bacteria and is a biodegradable thermoplastic. To produce PHB by
genetic engineering in plants, genes from the bacterium Alcaligenes
eutrophus that encoded the two enzymes required to convert
acetoacetyl-coenzyme A to PHB were placed under transcriptional
control of the cauliflower mosaic virus 35S promoter and introduced
into Arabidopsis thaliana. Transgenic plant lines that contained both
genes accumulated PHB as electron-lucent granules in the cytoplasm,
nucleus, and vacuole; the size and appearance of these granules were
similar to the PHB granules that accumulate in bacteria.
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