CTR1 caps, chromosome 5

[Shawn C. Baker]

In article <v01510101abc51682eb2f@[192.93.80.219]>
lgodiard at TOULOUSE.INRA.FR (Laurence Godiard) writes:

> Hi netters !
> 
> I would like to have some details about CTR1 Caps marker localize on top of
> chromosome 5. I tried to amplify from La-er and Col-0 DNA, and I got a 3.4
> kb faint product only with La-er (annealing temperature at 56 C), and with
> Col-0 a 0.8 kb product (should be a 3.4 kb fragment) that has a Bcl-1 site,
> enzyme recommended to give a polymorphism with CTR1 in the new Caps list.
> If anybody worked with this Caps marker, I would be glad to share his
> knowledge about.
> Thanks !
> 
> Laurence Godiard.
> 
> 

Hmm...  It seems like if these two phenotypes (3.4 vs. 0.8 kb bands)
are consistent, you have something better than a CAP - no digestion
necessary!

--
Shawn C. Baker              Division of Biological Sciences
scbaker at ucdavis.edu         Section of MCB (Gasser Lab)
(916) 752-3111              University of California - Davis
                            Davis, CA  95616