in planta transformation

P. Fobert pfobert at
Wed Aug 23 18:13:47 EST 1995

Hi, I'm sorry to post yet another query on this topic, but here 
we go...

1.  Has anyone assessed so-called wide-host range Agrobacterium 
strains such as EHA 101/105 for in planta transf?

2. What level of L-PPT (BASTA) should one use on germinating 
Arabidopsis seeds? Our pat gene is under control of double 35S 
promoter as well as a translatinal enhancer. (I apologize if 
this has been previously answered, I'm relatively new to this 

Thank you,  


Pierre Fobert (pfobert at
Research Officer
Plant Biotechnology Institute		
National Research Council of Canada	
110 Gymnasium Place				
Saskatoon, Sask

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