proteins

Sandy S. Berry-Lowe slblowe at ELCAT.UCCS.EDU
Wed Feb 8 13:10:11 EST 1995


Dear Arab netters, 
	Does anyone have a good procedure for Arabidopsis proteins on
a 2 d gel? We have made whole cell extracts-they work quite well on
SDS gels and westerns but usually don't even run into the ief gel when
we are trying 2ds. We are presently using the Bio-Rad mini gel system
and recipes. I would appreciate receiving any info from anyone who
has tried this successfully.
	I am also interested in talking to someone who has put 2
proteins into an expression system. Does it matter which order the
2 proteins are put in? What about any flanking sequence, particularly
on the 5' end of the 2nd gene? Will its ribosomal binding site be
used? If anyone could give me any advice on either of these two
questions, please answer me directly.
Sandy Berry-Lowe
Univ. Colorado

slblow at uccs.edu



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