in situ hybridization/fixing of sections to glass slides
peterswi at PILOT.MSU.EDU
Fri Feb 17 16:25:18 EST 1995
I am doing in situ hybridization with paraffin embedded plant sections. During
the hybridization step with a 50% formamide solution most of the sections fall
off. Therefore I would like to fix the sections to the glass slides according
to the method described in Tourtelotte et al. (1987), where aminopropyl-
triethoxysilane precoated slides are activated with glutaraldehyde for covalent
binding of the sections.
Does anybody have experience with this fixation method using plant tissue
Alternatively, does anybody have experience with commercially sold precoated
Any suggestions would kindly be appreciated.
peterswi at pilot.msu.edu
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