measuring cells in hypocotyls

[STEPHEN A ROLFE]

Dear netters,

I have an undergraduate project student in the lab who is examining 
factors affecting hypocotyl elongation in Arabidopsis.  Does anyone 
have a good staining protocol so that cells within the hypocotyl can 
be visualised easily?  The aim is to distinguish between cell 
expansion and cell division so we wish to be able to measure both 
cell number and cell length.

Thanks in advance for your help

Steve Rolfe
University of Sheffield
Department of Animal and Plant Sciences
P.O. Box 601
University of Sheffield
Sheffield
UK

Tel: (+44) 742 824115
Fax: (+44) 742 760159
e-mail: s.rolfe at sheffield.ac.uk