measuring cells in hypocotyls

Tony Travis ajt at rri.sari.ac.uk
Sun Feb 26 05:06:39 EST 1995


STEPHEN A ROLFE (S.Rolfe at sheffield.ac.uk) wrote:
: Dear netters,

: I have an undergraduate project student in the lab who is examining 
: factors affecting hypocotyl elongation in Arabidopsis.  Does anyone 
: have a good staining protocol so that cells within the hypocotyl can 
: be visualised easily?  The aim is to distinguish between cell 
: expansion and cell division so we wish to be able to measure both 
: cell number and cell length.

Try 2.5g borax + 2.5g Toluidine blue in 100 ml water.

For more details see Travis, A.J., Murison, S.D.M. and Chesson, A.
(1993)  Estimation of plant cell wall thickness and cell size by image
skeletonization, J. Ag. Sci., 120, 279-287.  You may also be interested
in the image analysis methods we used.

	Tony.
--
Dr. A.J.Travis,                       |  JANET: <ajt at uk.ac.sari.rri>
Rowett Research Institute,            |  other: <ajt at rri.sari.ac.uk>
Greenburn Road, Bucksburn,            |  phone: +44 (0)224 712751
Aberdeen, AB2 9SB. UK.                |    fax: +44 (0)224 716687


--
Dr. A.J.Travis,                       |  JANET: <ajt at uk.ac.sari.rri>
Rowett Research Institute,            |  other: <ajt at rri.sari.ac.uk>
Greenburn Road, Bucksburn,            |  phone: +44 (0)224 712751
Aberdeen, AB2 9SB. UK.                |    fax: +44 (0)224 716687



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