alternative carbon sources

Leonard N. Bloksberg bloksber at pilot.msu.edu
Fri Jan 20 13:53:00 EST 1995


In Article <n1421525964.53183 at raven.rutgers.edu> "zchen at OCELOT.RUTGERS.EDU ("Chen, Zhixiang")" says:
> Hi: 
> Sucrose has been commonly used as a carbon source and osmotic regulator for
> plant cell culture.  Since sucrose increases the background levels of an assay
> we are doing, I am interested to know whether there are alternative
> carbohydrate sources which can be used for tobacco cell suspension culture. If
> there are such suitable sources, I would appreciate knowing how they can
> substitute for sucrose (e.g. in what combination if more than one are
> required, at what concentrations etc).
> 
> Zhixiang Chen
> Waksman Institute
> Rutgers University
> zchen at mbcl.rutgers.edu    
> 
.
I've replaced 3% sucrose with 2% glucose in tobacco and tomato tissue culture
with success.  Try asking Dr. C.K. Chin across campus.  He was my first 
tissue culture instructor when I was an undergrad @ RU.  Good luck
.
Leonard N. Bloksberg
bloksber at pilot.msu.edu
.
.



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