Please has anyone used this vector pART27 for root transformation in Arabidopsis? If so what concentration of kan did you use? Our transformants? are dying at 50ug/ml selection. The kanr gene is from pGA463. Thanks Liz Dennis Liz Dennis CSIRO Division of Plant Industry GPO Box 1600 Canberra 2601 Australia Tel +61-6-2465061 Fax +61-6-2465000