GFP Splice Site Responses

Paul Kitts paulki at clontech.com
Wed Jun 14 13:44:15 EST 1995


Mark Guiltinan quoted a message from Joan Krochko:
>
> Mark,
> 
> The information that I have received form Clontech states that the 
> cryptic intron (84 bp) in the GFP is:
> 
>     AG|GUAUUGA --------UCAUGGCAG|AC
>     
> According to Paul Kitts at Clontch, Jim Hasseloff changed the 5' 
> splice site and elevated the CG content of theis region.  This 
> greatly improved the fluorescence in Arabidopsis under the 35S 
> promoter. 

[snip]

I would like to caution that this is taken from my notes on Jim 
Haseloff's presentation at the Fluorescent Protein meeting in March. 
I suggest before acting on this information you check it with Jim 
Haseloff.

-= Paul =- 

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