GFP Splice Site Responses
paulki at clontech.com
Wed Jun 14 13:44:15 EST 1995
Mark Guiltinan quoted a message from Joan Krochko:
> The information that I have received form Clontech states that the
> cryptic intron (84 bp) in the GFP is:
> AG|GUAUUGA --------UCAUGGCAG|AC
> According to Paul Kitts at Clontch, Jim Hasseloff changed the 5'
> splice site and elevated the CG content of theis region. This
> greatly improved the fluorescence in Arabidopsis under the 35S
I would like to caution that this is taken from my notes on Jim
Haseloff's presentation at the Fluorescent Protein meeting in March.
I suggest before acting on this information you check it with Jim
-= Paul =-
! Paul Kitts !
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