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postdoc position

SBY1RO at rulsfb.LeidenUniv.nl SBY1RO at rulsfb.LeidenUniv.nl
Wed Mar 1 08:52:47 EST 1995

   Available from October/November 1995 within the EU Program:
                  Human Capital and Mobility


                    on the project:

          Molecular mechanisms of auxin action:
          from primary perception to gene expression


For this position we are looking for an enthusiastic non-dutch
EC citizen who has recently earned her/his Ph.D. degree in a
molecular biology/genetics subject, preferably has worked with
Arabidopsis and has affinity with plant tissue culture.

The appointment will be for the period of 2 years and the
candidate will work in section 2 (developmental genetics) of the
institute under supervision of Dr Remko Offringa and
Prof Dr Paul J.J. Hooykaas.

Please submit CV (including references) and list of publications
within two weeks to:

Dr. Remko Offringa

Email:         SBY1RO at RULSFB.LeidenUniv.nl
Fax:           31-71-274999
Mail address:  Leiden University
               Institute of Molecular Plant Sciences
               Clusius Laboratory, Wassenaarseweg 64
               2333 AL Leiden, The Netherlands

The project concerns the identification of genes coding for
the different components of the auxin signal transduction
pathways in Arabidopsis thaliana. Different approaches are
being followed in order to achieve this goal:

1) Recently auxin inducible promoter activities have been
isolated from Arabidopsis thaliana. Transgenic Arabidopsis
lines carrying the auxin responsive promoter fused to the á-
glucuronidase coding region (GUS) will be used to study gene
regulation in more detail. This will be done in planta as well
as in a cell suspension-, protoplast- or root culture system.
We are now looking for possibilities to use the GFP encoding
gene as a reporter instead of or next to GUS.

2) Constructs will be made in which the auxin-responsive
promoter is fused to either a negative or a positive selective
gene and Arabidopsis will be transformed with these. The
transgenic lines will be used to isolate mutants (T-DNA or EMS
induced) in which auxin-response of the promoter is either
inhibited or up-regulated, respectively.

We foresee that by following the above-mentioned approaches
clones will become available coding for sofar unknown
components of the auxin signal transduction pathways. These
may become very useful for the development of systems for
growth control of plants.

About the Institute of Molecular Plant Sciences:

The institute concentrates research groups active in the
molecular plant sciences at Leiden and operates at the
interface of chemistry and biology. Through molecular
approaches, complex problems of plant development and of the
reactions of plants to changing environmental conditions are
studied focusing on intracellular and environmental signalling
systems directing plant growth, defence and development.
Fundamental research on plant-microbe interactions
traditionally forms an important part of the research in the
institute.  The institute consists of 6 sections that are
founded in the traditional plant disciplines i.e.:

1) molecular biology ( prof. dr. RA Schilperoort),
2) developmental genetics (prof. dr. PJJ Hooykaas),
3) virology (prof. dr. JF Bol),
4) microbiology (prof. dr. BJJ Lugtenberg),
5) physiology (prof. dr. JW Kijne)
6) cell biology (prof. dr. ThM Konijn).

The research themes are of a fundamental nature but they
relate to problems and needs of agriculture and industry.
Moreover, strategic research is done in collaboration with
both public and private institutions (national and

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