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BAC library construction, I need help!

Thu Mar 16 11:56:31 EST 1995

  I am a second year masters student at McGill University. I have been
attempting to construct a High Molecular Weight (HMW) DNA library using
BAC but have been completely unsuccessful at producing clones. I know
the problem is with ligating the HMW DNA to the vector. I have already
checked the ligase and my vector and neither of those appears to be the
problem. The problem seems to be restricted to the HMW DNA. Does anyone
have any advice on this subject? Your help would be greatly appreciated.

        Jennifer Syme

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