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Fri Mar 17 11:37:27 EST 1995

Dear netters
Here goes the summary of responses on REMI (restriction enzyme mediated
integration). I apologize for delay in posting this.
It appears that there is a considerable interest in this technique for 
insertional mutagenesis in plants, although not much has been done.
References follow:

General description of the technique:

Integration of DNA fragments by illegitimate recombination in 
Saccharomyces cerevisiae.  Proc.Natl. Acad. Sci USA 88:7585-7589. 1991
RH Schiestl and TD Petes

Tagging developmental genes in Dictyostelium by restriction 
enzyme-mediated integration of plasmid DNA. Proc. Natl. Acad. Sci. USA 
89:8803-8807. 1992. A Kuspa and WF Loomis

One recent application of REMI in slime mold:

Dynes et al, 
Genes and Devel., 8:948-958, 1994

And the only (?) plant paper describing first step towards development of
REMI in plants:

Alteration of gene expression by restriction enzymes electroporated into 
plant cells. Mutation Res. 302:75-82. 1993. M Ashraf, M Atschuler, S 
Galasinski,and TD Griffiths.

Thanks to all who replied!!!
Dima Belostotsky

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