DIG-labeling, lambda phage (clones from Meyerowitz)

Carsten Koehn cereka at fub46.zedat.fu-berlin.de
Sun Mar 19 13:41:50 EST 1995


Has someone a reliabel!! protocol for the hybridization of southern blots 
using the digoxigenin system? I am working with the lambda clones from 
Meyerowitz and colleages. As the insert is large (up to 7 kb) and I have 
no information about the sequence, labeling with a PCR reaktion does not 
work. 
I am grateful for every hint
Carsten Koehn

email: cereka at fub46.zedat.fu-berlin.de




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