DAPI Staining

Shigeki 'LARGE' NAKAYAMA shignak at abr.affrc.go.jp
Sun Mar 26 03:43:15 EST 1995


In article <9503242333.AA08543 at mendel.Berkeley.EDU>, 
nohad at MENDEL.BERKELEY.EDU wrote:

> I am looking for a procedure to stain Arabidopsis endosperm nuclei,
with
> DAPI  or other  flourescent dye ( that would not decay quickly). I 
would
> like to use this  to quantitate DNA amounts and estimate ploidy  in
these
> nuclei.

Hi,
Although I am not sure about this matter, DAPI is used as 
the base specific dye for chromosomes (AT-rich).
How about EtBr? It isn't base specific dye, is it?

!(^_^)!|(-_^)||(-_-)|m(_ _)m<(   )>w( - )w|(-_-)||(^_-)|!(^_^)!
                                               Shigeki Nakayama
                                           NIAR, Tsukuba, Japan
                                     shignak at ss.abr.affrc.go.jp
!(^_^)!|(^_-)||(-_-)|w( - )w<(   )>m(_ _)m|(-_-)||(-_^)|!(^_^)!



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